Molecular Plant (MP) Best of 2021 新鲜出炉！根据2021年MP所发表文章的下载阅读量、引用率和专家评语等多方面综合因素, 我们评选出12篇MP 2021年度最佳论文。热烈祝贺入选MP Best of 2021的12篇论文的作者们！并衷心感谢MP的作者、审稿人和读者们，期待大家科研工作乘风破浪、再创佳绩！
论文的题目、作者、链接和简单介绍如下，欢迎大家下载、阅读和讨论。在线阅读原文可访问：
https://molecularplant-bestof2021.elsevierdigitaledition.com/
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www.cell.com/molecular-plant
一．前瞻性综述
1.Single-Cell Transcriptome Analysis in Plants: Advances and Challenges
https://doi.org/10.1016/j.molp.2020.10.012
Single-cell RNA sequencing has ushered in a new era of transcriptome analysis on a cell-by-cell basis. Application of this technology to plant biology has provided important insights into cell-to-cell transcriptional heterogeneity but also posed some unique challenges for plant single-cell researchers. Further development of both wet-lab techniques andin silico methods is necessary to unleash its full potential in plant research and breeding.
2.Protein Quality Control in Plant Organelles: Current Progress and Future Perspectives
https://doi.org/10.1016/j.molp.2020.10.011
Accumulation of misfolded proteins in plant organelles can cause dysfunctions that lead to developmental defects and impaired environmental stress tolerance. This review summarizes recent advances in the understanding of the mechanisms that safeguard protein quality in the ER, chloroplasts, and mitochondria, and highlights common mechanisms shared by these organelles that ensure protein homeostasis in plant cells.
3.Plant cytochrome P450 plasticity and evolutionhttps://doi.org/10.1016/j.molp.2021.06.028
Cytochrome P450 enzymes play critical roles in regulation and diversification of plant metabolism. This review article provides an updated annotation of the plant cytochrome P450 families within the plant lineage and discusses molecular features and dynamics of cytochrome P450 plasticity and evolution. This is presented along with specific examples from various biosynthetic pathways.
二．研究论文
4. Hybrid speciation via inheritance of alternate alleles of parental isolating genes
https://doi.org/10.1016/j.molp.2020.11.008
Using integrated approaches, the authors reveal that reproductive isolation originated in one homoploid hybrid plant species (Ostryopsis intermedia) through the inheritance of alternate alleles at genes that determine parental premating isolation (flowering time and habitat adaptation, i.e., iron tolerance). Their findings and further modeling results suggest that homoploid hybrid speciation can occur easily via the inheritance of alternate, pre-existing, parental reproductive isolations.
5. Genome- and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus
https://doi.org/10.1016/j.molp.2020.12.003
Seed oil content is an important and complex trait of the oil crop Brassica napus. This study identifies the quantitative trait loci (QTLs) and candidate genes associated with seed oil content in B. napus using comprehensive genome- and transcriptome-wide association studies.
6.Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signal to control polyadenylation site choice in liquid-like nuclear body
https://doi.org/10.1016/j.molp.2021.01.014
CPSF30-L was characterized as an Arabidopsis N6-methyladenosine (m6A) reader whose m6A-binding function is required for the floral transition and abscisic acid (ABA) response. The m6A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies, where CPSF30-L mainly recognizes the m6A-modified far-upstream element to control polyadenylation-site choice. Disruption of CPSF30-L lengthens the 3′ UTR of three phenotypically related genes, thereby accelerating mRNA degradation and causing late flowering and ABA hypersensitivity.
7. A transceptor-channel complex couples nitrate sensing to calcium signaling in Arabidopsis
https://doi.org/10.1016/j.molp.2021.02.005
In this study, authors demonstrate that CNGC15 interacts with the nitrate transceptor NRT1.1 to constitute a molecular switch that either closes or opens the calcium influx channel (CNGC15) upon the formation or dissociation of the NRT1.1–CNGC15 complex. This leads to generation of a nitrate-specific calcium signature that is evoked upon a rise in nitrate levels. Thus, this study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor–channel complex assembly to couple nutrient status to a specific Ca2+signature.
8.Two Gap-free Reference Genomes and a Global View of the Centromere Architecture in Rice
https://doi.org/10.1016/j.molp.2021.06.018
This study reports two gap-free reference genomes for Oryza sativa xian/indica varieties ZS97 and MH63, which are used as a model system for studying rice heterosis and yield. Further genomic analyses show that the architectures of functional centromeres are extensively variable at the sequence level. These two gap-free rice genomes provide a new standard for assembling reference genomes of other plant species.
9.The INO80 chromatin remodeling complex promotes thermomorphogenesis by connecting H2A.Z eviction and active transcription in Arabidopsishttps://doi.org/10.1016/j.molp.2021.07.001
This study reports that a (PIF4)-(INO80-C)-(COMPASS-like)-(transcription elongator) module integrates H2A.Z eviction, H3K4me3 deposition, and active transcription to regulate high-temperature-induced plant responses. The study reveals an epigenetic mechanism underlying plant developmental plasticity in response to environmental chan
10.Persulfidation-induced Structural Change of SnRK2.6 Establishes Intramolecular Interaction between Phosphorylation and Persulfidation
https://doi.org/10.1016/j.molp.2021.07.002
H2S-linked persulfidation altered SnRK2.6 structure, which effectively improved the transfer efficiency of phosphate groups, resulting in the enhanced phosphorylation level of SnRK2.6. Meanwhile, the phosphorylation status of S267 positively regulated the persulfidation of SnRK2.6.
三、资源、方法性论文
11.Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression in Arabidopsis roots at the single-cell level
https://doi.org/10.1016/j.molp.2021.01.001
To gain a deeper understanding of the regulatory mechanisms controlling plant gene expression, we report the use of isolated Arabidopsis thaliana root nuclei to establish biologically meaningful transcriptomes and profiles of chromatin accessibility at the single-cell level. The comprehensive integration of these datasets reveals that the differential chromatin accessibility plays a critical role in controlling plant gene activity.
12.Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture
https://doi.org/10.1016/j.molp.2021.07.010
Using genome editing to obtain heritable genetic modified plants currently relies on conventional genetic transformation and plant regeneration procedures in most cases. This study reports on a Barley stripe mosaic virus–based sgRNA delivery vector system, which could efficiently induce heritable genome editing in hexaploid wheat plants while bypassing the laborious tissue culture process.
转自：植物科学最前沿
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